wright s giemsa staining kit  (Servicebio Inc)

Journal: Asian Journal of Pharmaceutical Sciences

Article Title: Light-stimulated smart thermo-responsive constructs for enhanced wound healing: A streamlined command approach

doi: 10.1016/j.ajps.2025.101057

Figure Lengend Snippet: Capability of NIR-stimulated STS for wound infection prevention: (A) Schematic diagram of experimental design during a 7 d treatment period; (B) H&E, Ly6g, IL-6, and Giemsa staining; (C-E) Quantified analysis of Ly6g expression, IL-6 expression and bacteria number.

Article Snippet: Carbodiimide (EDC) was procured from TCI AMERICA (China); N-hydroxysuccinimide (NHS) was obtained from Aladdin Scientific (China); Phosphate-buffered saline (PBS) and paraformaldehyde were purchased from Biosharp (China); Gelatin sourced from porcine skin, 1,6-hexanediamine and dopamine (DA) were obtained from Sigma-Aldrich (USA); Poly- l -lactic acid (PLLA; Mw = 100,000 Da) and PLCL (LA/CL 50:50, IV 2.9 dl/g) were procured from Jinan Daigang Biomaterials (China); 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) was obtained from Chembee (China); CuCl 2 was obtained from Sinopharm Chemical Reagent (China); Tris–HCl buffer was purchased from Shanghai Yuan Mu Biotechnology (China); Bicinchoninic acid (BCA) reagent and CCK-8 were procured from Beyotime Biotechnology (China); Lys was obtained from Shanghai yuanye Bio-Technology (China); PLys (Mw ∼5,000) was obtained from Macklin (China); Luria-Bertani (LB) agar, Triton X-100 and goat serum were procured form Solarbio (China); Rat bFGF recombinant protein was purchased from PeproTech (USA); Human umbilical vein endothelial cells (huvECs) were obtained from the National Stem Cell Translational Resource Center of China; human Endothelial Cell Medium was obtained from ScienCell (USA); Mouse L929 fibroblast cells were purchased from Shanghai YanJin Biotech Co. Ltd. (China); Dulbecco's Modified Eagle (DMEM) medium was obtained from Biological Industries (Israel); Tetramethylrhodamine (TRITC)-Phalloidin and 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI) were obtained from Yeasen Biotechnology (China); Rabbit anti-mouse Proliferating Cell Nuclear Antigen (PCNA) monoclonal antibody was obtained from Abcam (UK); Fluorescein (FITC)-conjugated affinipure goat anti-rabbit IgG (H+L) antibody was obtained from Proteintech (USA); Total RNA isolation kit was purchased from Biozol (China); FastKing RT kit (with gDNase) and Super Real PreMix Plus (SYBR Green) reagent kit were got from Tiangen (China); All the primer sequences were purchased from Sangon Biotech. (China); Male healthy Sprague Dawley (SD) rats were procured from Beijing Vital River Laboratory Animal Technology Co., Ltd. (China); 3′-diaminobenzidine (TAB), hematoxylin and eosin (H&E), Masson's trichrome, Wright's Giemsa staining kit, rabbit anti-interleukin-6 (IL-6) antibody, CY3-labeled goat anti-rabbit IgG, rabbit anti-lymphocyte antigen 6 complex locus G6D (Ly6g) antibody, and HRP-labeled goat anti-rabbit IgG were purchased from Servicebio (China).

Techniques: Infection, Staining, Expressing, Bacteria